In late time, the interest for the restorative plants is expanding in both creating and created nations because of developing acknowledgment of common items as being non-poisonous, without reactions, effortlessly accessible, and of reasonable costs. Conventional medication, being a huge component in the social patrimony, still remains the fundamental plan of action for a vast dominant part of individuals for treating medical issues like cancer and so on [1]. Roughly 80% of the populace utilizes it as a cure. To manage the advancement of wellbeing and treating illnesses generally use of bioactive organic build-ups has profound roots in man's history and is all-encompassing which is increasing more prominent consideration and prominence in numerous locales of the world [2, 3]. Plants and plant items are utilized in treating jungle fever, looseness of the bowels, consumption, gonorrhoea, stomach issue, and different irresistible sicknesses. Endeavours of researchers in building up biogenic fixings with promising antimicrobial movement are yielding productive outcomes step by step [4]. Regular antimicrobials contain dynamic mixes of bioactive mixes to battle against the obstruction of microbes and save probiotic species in this way, they are alluring choices in numerous malady models [5,6]. The citrus and non-citrus natural products are such a potential specialist where non-citrus organic products incorporate banana (Musa sharpen), pomegranate (Punicagranatum), sapodilla (Manilkarazapota), apple (Malusdomestica), pineapple (Ananascomosus), cucumber (Cucumissativus), guava (Psidiumguajava) and so forth and the citrus organic products incorporate lime (Citrus aurantiifolia), pomelo (Citrus maxima), kaffir lime (Citrus hystrix) et cetera. Several health benefits of citrus fruits have been described in part to Vitamin C, which is  the most common vitamin found in fruits and vegetables [7]. A plethora of Citrus species are having potential antimicrobial activity followed by different secondary metabolites or phytochemicals [8]. These phytochemicals are believed to be responsible for wide range of protective health benefits including anti oxidative, anti-infl ammatory, antitumor, and antimicrobial activities [9,10]. Pineapples are the most recent and weirdest weapon to develop in the worldwide fight against superbugs which are anticipated to slaughter up to 10 million individuals per year by 2050. Moreover, potential enzyme named Bromelain, obtained from pineapple, is a strong anti-enter pathogenic agent that showed most efficacies against several pathogenic bacteria. Diminishing effectiveness and opposition of pathogens to antimicrobial medications made the inquiry of another antimicrobial operator as a vital system for the foundation of elective treatments is troublesome dealing with contaminations. E.coli, Enteropathogenic E. coli, Enterotoxigenic E. coli, S. aureus, V. parahaemolyticus, and Salmonella spp. are the real reasons for intestinal diseases. Pineapples, Lime, Kaffir lime, and Pomelo are heavenly tropical natural products that have been praised for quite a long time for their particular and exceptional taste, as well as for their marvelous medical advantages. Because of the report of expanding advancements of medication obstruction in human pathogen and in addition unfortunate symptoms of certain antimicrobial specialists, it is important to scan for new operators which are better, less expensive, and without reaction for treating irresistible sicknesses particularly in creating healthy nations. On the other hand as the wellbeing parts of substance or manufactured sustenance added substances are being scrutinized, the interest for normally happening additives has been expanded around the world [11]. The investigation of novel antimicrobial specialists from regular assets comprehensive of plant or plant-based items (flavor or herbs) and others has been utilized chiefly to cure disease, nourishment security, and sustenance preservation reason since numerous years back [12,13]. Overall aimless employments of anti-infection agents to control pathogenic microscopic organisms have prompted the spread of the safe strains of microorganisms making treatment of bacterial diseases by these medications troublesome. In this way, researchers are looking for safe elective treatments against these hazardous and even perilous pathogens. Plants create numerous phytochemicals having antimicrobial properties. These might be utilized as normal medications which are for the most part non-toxic and more affordable than the manufactured medications. In this investigation, four distinctive antimicrobial characteristics contain assets, for example, non- citrus natural products incorporate pineapple (Ananascomosus), and the citrus organic product incorporates lime (Citrus aurantiifolia), pomelo (Citrus maxima), kaffir lime (Citrus hystrix) were broken down for antimicrobial adequacy. Finally, in this endeavor, we tried to discover the antimicrobial properties of the juices of some basic eatable Citrus and non-citrus organic products against six pathogenic microbes.

Cultures of six pathogenic bacteria namely- Gram-positive S. aureus and other is Gram-negative E. coli, Enteropathogenic E. Coli (EPEC), Enterotoxigenic E. Coli (ETEC), V. parahaemolyticus, Salmonella spp. were obtained from the institutional microbiology laboratory and were stored in a refrigerator. Antimicrobial properties of fresh fruit juices were evaluated by agar well diffusion method. Briefly, petriplates containing nutrient medium composed of peptone, beef extract, sodium chloride, agar and distilled water were prepared for the evaluation of antimicrobial activities of fruit juices. 100µl of inoculum of each bacterium under our study was uniformly spreaded on the nutrient agar lawn in separate petriplates with the help of a sterilized glass spreader and the plates were then incubated for 30 minutes in the room temperature inside a laminar hood. Following this, with the help of a sterile cork, wells having the diameter of 6mm were bored in the plates. 50 µl of fresh  crude and diluted fruit juice samples, distilled water and antibiotic disc erythromycin & penicillin were poured separately into the wells. Distilled water and antibiotic acted as negative and positive controls respectively. After 10 minutes of incubation at room temperature all the plates were transferred to an incubator and kept inside it at 37°C for 24 hours. Following the incubation, plates were checked for the presence of zones of inhibition (ZOI). Diameters of the observed ZOIs were measured in mm and the results were recorded by taking photographs of the ZOIs on the plates.

Fruit juices are valuable and readily available resources for primary health care and complementary health care system, undoubtedly fruit juices containing substances of medicinal value that have yet to be discovered, though large number of fruit juices are constantly being screened for their antimicrobial effect, these fruits may prove to be a rich source of compounds with possible antimicrobial activities. In the present study agar well diffusion method was used here in order to get antimicrobial activity of fresh citrus and non-citrus fruit juice against six bacterial strains among one is Gram-positive S. aureus and others are Gram-negative E. coli, Enteropathogenic E. coli (EPEC), Enterotoxigenic E. coli (ETEC), V. parahaemolyticus, Salmonella spp. Also MIC measurement was done to know the minimum inhibitory concentration of fruit juices by OD measurement using UV. Spectrophotometer. The non-citrus fruit include banana (Musa acuminate), pomegranate (Punicagranatum), sapodilla (Manilkarazapota), apple (Malusdomestica), pineapple (Ananascomosus), cucumber (Cucumissativus), and guava (Psidiumguajava) whereas, only pineapple can show antimicrobial activity. The citrus fruit include lime (Citrus aurantiifolia), pomelo (Citrus maxima), kaffir lime (Citrus hystrix) whereas, all showed antimicrobial activity. Results of our study showed that juices of four fruits exhibited inhibitory actions against the Gram-positive S. aureus and other is Gram-negative E. coli, Enteropathogenic E. coli (EPEC), Enterotoxigenic E. coli (ETEC), V. parahaemolyticus, Salmonella spp. Table no.1-4 represented the antimicrobial activity of various concentrations (100%, 75%, 50%, and 25 %.) of fresh Lime, Kaffir lime, Pineapple and Pomelo fruit juices respectively. The minimum inhibitory concentration (MIC) of various concentration of Lime, Kaffir lime, Pineapple and Pomelo juices based on OD measurement (mean±sd) represented in Table 5-8. The zones of inhibition against various selected pathogens were measured in mm (mean±sd). Zone of inhibition were seen against all species confirming the antimicrobial activity of fresh Lime, Kaffir lime, Pineapple and Pomelo juices (Figures 1-4). The fresh crude lime fruit juice produced the highest antimicrobial activity against S. aureus (21.33±1.155mm) followed by V. parahaemolyticus (20.33±0.577mm), E. coli (18.00±1.00mm), EPEC    (18.333±0.577    mm),    ETEC    (17.667±0.577mm), & Salmonella spp. (14.00±1.00 mm) (Table 1). The fresh crude kaffir lime fruit juice produced the highest antimicrobial activity against EPEC    (23.00±1.00mm)    followed    by    V.   parahaemolyticus (19.33±1.154mm),     ETEC      (16.667±0.577mm),     S.      aureus (19.67±0.577mm) & E.coli (16.667±1.528 mm) (Table 2).